Article
Haplotyping of Heterozygous SNPs in Genomic DNA Using Long-Range PCR.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Arbeithuber Barbara, Heissl Angelika, Tiemann-Boege Irene
Abstract excerpt
To study meiotic recombination products, cis- or trans-association of disease polymorphisms, or allele-specific expression patterns, it is necessary to phase heterozygous polymorphisms separated by several kilobases. Haplotyping using long-range polymerase chain reaction (PCR) is a powerful, cost-effective method to directly obtain the phase of multiple heterozygous sites with standard laboratory equipment in a...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.