Article
Comparison of proliferation and rapid cytokine induction assays for flow cytometric T-cell epitope mapping.
Cytometry. Part A : the journal of the International Society for Analytical Cytology - 1 Mar 2003
Tesfa Lydia, Volk Hans-Dieter, Kern Florian
Abstract excerpt
BACKGROUND: T-cell epitope mapping by flow cytometry based on rapid ex vivo peptide-specific cytokine induction in T cells is very efficient and time saving compared with traditional assays. We investigated whether the same epitopes could be identified by proliferation studies. METHODS: An assay based on rapid interferon-gamma induction in T cells (6 h of ex vivo stimulation) was run in parallel with a...
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