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Article

Analysis of cellular phenotype during in vitro immunization of murine splenocytes for generating antigen-specific immunoglobulin.

Journal of bioscience and bioengineering - 1 Mar 2013

Inagaki Takashi, Yoshimi Tatsunari, Kobayashi Satoshi, Kawahara Masahiro, Nagamune Teruyuki

Abstract excerpt

Although various in vitro immunization methods to generate antigen-specific antibodies have been described, a highly effective method that can generate high-affinity immunoglobulins has not yet been reported. Herein, we analyzed a cellular phenotype during in vitro immunization of murine splenocytes for generating antigen-specific immunoglobulins. We identified a combination of T cell-dependent stimuli (IL-4,...

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Identifiers

PMID: 23177212
DOI: 10.1016/j.jbiosc.2012.10.008

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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