Article
Simultaneous cytometric analysis of (auto)antigen-reactive T and B cell proliferation.
Immunobiology - 1 Dec 2002
Schneider Sandra, Bruns Anne, Moewes Beate, Holzknecht Barbara, Hausdorf Gerd, Riemekasten Gabriela, Radbruch Andreas, Hiepe Falk, Thiel Andreas
Abstract excerpt
The detection and characterization of (auto)antigen-specific lymphocytes, both B and T cells, is essential to investigate immunopathologic mechanisms. Our aim was to perform a CFSE (Carboxyfluorescein diacetate succinimidyl ester)-based cytometric analysis of peripheral blood mononuclear cells (PBMC) proliferating in response to antigenic provocation. CFSE-labeled PBMC were stimulated with a superantigen (SEB), a...
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