Article
The duration of in vitro stimulation with recall antigens determines the subset distribution of interferon-gamma-producing lymphoid cells: a kinetic analysis using the Interferon-gamma Secretion Assay.
Journal of immunological methods - 1 Jun 2005
Desombere I, Clement F, Rigole H, Leroux-Roels G
Abstract excerpt
Analyses of cellular immune responses during natural infections and following vaccination with established or candidate vaccines are becoming increasingly important and so are the research tools used to achieve this goal. During a recent evaluation of the analytical performance characteristics of one of these techniques, the interferon-gamma secretion assay, we noticed that following overnight incubation of PBMC...
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