Article
Sendai virus-induced IFN-alpha production analysed by immunocytochemistry and computerized image analysis.
Scandinavian journal of immunology - 1 Oct 1996
Akerlund K, Björk L, Fehniger T, Pohl G, Andersson J, Andersson U
Abstract excerpt
IFN-alpha production in Sendai virus-stimulated human buffy coat cultures could readily be demonstrated in individual cells at a protein level by the use of a novel immuno-enzymatic staining procedure. A distinctive rounded, juxtanuclear staining pattern was generated in producer cells by the accumulation of the intracellularly synthesized IFN-alpha in the Golgi stacks. The technology is based upon acquiring a...
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