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Article

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening.

Journal of visualized experiments : JoVE - 1 Apr 2016

Viña-Gonzalez Javier, Gonzalez-Perez David, Alcalde Miguel

Abstract excerpt

Directed evolution in Saccharomyces cerevisiae offers many attractive advantages when designing enzymes for biotechnological applications, a process that involves the construction, cloning and expression of mutant libraries, coupled to high frequency homologous DNA recombination in vivo. Here, we present a protocol to create and screen mutant libraries in yeast based on the example of a fungal aryl-alcohol...

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Identifiers

PMID: 27077451
DOI: 10.3791/53761
PMCID: PMC4841332

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