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Article

DNA shuffling method for generating highly recombined genes and evolved enzymes.

Nature biotechnology - 1 Apr 2001

Coco W M, Levinson W E, Crist M J, Hektor H J, Darzins A, Pienkos P T, Squires C H, Monticello D J

Abstract excerpt

We introduce a method of in vitro recombination or "DNA shuffling" to generate libraries of evolved enzymes. The approach relies on the ordering, trimming, and joining of randomly cleaved parental DNA fragments annealed to a transient polynucleotide scaffold. We generated chimeric libraries averaging 14.0 crossovers per gene, a several-fold higher level of recombination than observed for other methods. We also...

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PMID: 11283594
DOI: 10.1038/86744

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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