Article
Initiation factor eIF5B catalyzes second GTP-dependent step in eukaryotic translation initiation.
Proceedings of the National Academy of Sciences of the United States of America - 24 Dec 2002
Lee Joon H, Pestova Tatyana V, Shin Byung-Sik, Cao Chune, Choi Sang K, Dever Thomas E
Abstract excerpt
Initiation factors IF2 in bacteria and eIF2 in eukaryotes are GTPases that bind Met-tRNA(i)(Met) to the small ribosomal subunit. eIF5B, the eukaryotic ortholog of IF2, is a GTPase that promotes ribosomal subunit joining. Here we show that eIF5B GTPase activity is required for protein synthesis. Mutation of the conserved Asp-759 in human eIF5B GTP-binding domain to Asn converts eIF5B to an XTPase and introduces an...
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