Article
Uncoupling of initiation factor eIF5B/IF2 GTPase and translational activities by mutations that lower ribosome affinity.
Cell - 27 Dec 2002
Shin Byung-Sik, Maag David, Roll-Mecak Antonina, Arefin M Shamsul, Burley Stephen K, Lorsch Jon R, Dever Thomas E
Abstract excerpt
Translation initiation factor eIF5B/IF2 is a GTPase that promotes ribosomal subunit joining. We show that eIF5B mutations in Switch I, an element conserved in all GTP binding domains, impair GTP hydrolysis and general translation but not eIF5B subunit joining function. Intragenic suppressors of the Switch I mutation restore general translation, but not eIF5B GTPase activity. These suppressor mutations reduce the...
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