Article
Further characterization of human fetal osteoblastic hFOB 1.19 and hFOB/ER alpha cells: bone formation in vivo and karyotype analysis using multicolor fluorescent in situ hybridization.
Journal of cellular biochemistry - 1 Jan 2002
Subramaniam M, Jalal Syed M, Rickard David J, Harris Steven A, Bolander Mark E, Spelsberg Thomas C
Abstract excerpt
We have previously generated an immortalized human fetal osteoblastic cell line (hFOB) using stably transfected temperature sensitive SV40 T-antigen (Harris et al. [1995a] J. Bone. Miner. Res. 10:178-1860). To characterize these cells for phenotypic/genotypic attributes desired for a good cell model system, we performed karyotype analysis by multicolor fluorescent in situ hybridization (M-FISH), their ability to...
Topics
- Animals
- Bone and Bones
- Cell Line
- Cells, Cultured
- Chromosomes
- Collagen
- Drug Combinations
- Genotype
- Humans
- In Situ Hybridization, Fluorescence