Article
Development and characterization of a conditionally immortalized human fetal osteoblastic cell line.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research - 1 Feb 1995
Harris S A, Enger R J, Riggs B L, Spelsberg T C
Abstract excerpt
We report the establishment of a human fetal osteoblast cell line derived from biopsies obtained from a spontaneous miscarriage. Primary cultures isolated from fetal tissue were transfected with a gene coding for a temperature-sensitive mutant (tsA58) of SV40 large T antigen along with a gene coding for neomycin (G418) resistance. Individual neomycin resistant colonies were screened for alkaline phosphatase...
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