Article
Functional properties of a conditionally phenotypic, estrogen-responsive, human osteoblast cell line.
Journal of cellular biochemistry - 1 Jun 1997
Bodine P V, Green J, Harris H A, Bhat R A, Stein G S, Lian J B, Komm B S
Abstract excerpt
Osteoblasts are established targets of estrogen action in bone. We screened 66 conditionally immortalized clonal human osteoblast cell lines for estrogen receptors (ERs) using reverse transcriptase-polymerase chain reaction (RT-PCR) analysis for ER alpha mRNA and transactivation of adenovirus-est...
Topics
- Aged
- Alkaline Phosphatase
- Antigens, Polyomavirus Transforming
- Calcitriol
- Cell Division
- Cell Line
- Cell Line, Transformed
- Estradiol
- Female
- Gene Expression
- Humans
- Osteoblasts
- Osteocalcin
- Phenotype
- Polymerase Chain Reaction
- Receptors, Estradiol
- Temperature