Article
Immortalization and characterization of bone marrow stromal fibroblasts from a patient with a loss of function mutation in the estrogen receptor-alpha gene.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research - 1 Apr 1998
Dieudonné S C, Xu T, Chou J Y, Kuznetsov S A, Satomura K, Mankani M, Fedarko N S, Smith E P, Robey P G, Young M F
Abstract excerpt
A male patient with abnormal postpubertal bone elongation was shown earlier to have a mutation in both alleles of the estrogen receptor, resulting in a nonfunctional gene. Marrow stromal fibroblasts (MSFs) derived from this patient were called HERKOs (human estrogen receptor knock outs), and in order to obtain continuous HERKO cell lines, they were immortalized using a recombinant adenovirus-origin-minus SV40...
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