Article
Trans-acting RNA inhibits tRNA suppressor activity in vivo.
RNA (New York, N.Y.) - 1 Jul 2002
Attardi Domenica Gandini, Tocchini-Valentini Glauco P
Abstract excerpt
We constructed two aptamers, each of which contains a 7-nt-long loop complementary to the anticodon loop of a suppressor tRNA. One of these aptamers can form a stable bimolecular complex with the suppressor tRNA in vitro and protects the 7 nt in the suppressor's anticodon loop from RNase S1. An Escherichia coli strain, carrying an amber mutation in the lac Z gene, produces beta-galactosidase only if the...
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