Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Comprehensive screening of amber suppressor tRNAs suitable for incorporation of non-natural amino acids in a cell-free translation system.

Biochemical and biophysical research communications - 19 Sept 2008

Taira Hikaru, Matsushita Yosuke, Kojima Kenji, Shiraga Kaori, Hohsaka Takahiro

Abstract excerpt

Incorporation of non-natural amino acids into proteins in response to amber or four-base codons is a useful technology for protein research. In the case of the amber codon, however, release factor 1 can competitively decode the same codon, and consequently inhibit the incorporation of non-natural amino acids. To improve amber codon-mediated incorporation, we carried out a comprehensive screening of amber...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acids
Share to Cell-Free System

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Codon, Terminator

Share to Protein Biosynthesis

Share to Protein Engineering

Share to RNA, Bacterial

Share to RNA, Transfer

Share to Suppression, Genetic

Source

Identifiers

PMID: 18634752
DOI: 10.1016/j.bbrc.2008.07.020

Genes

SDS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Comprehensive screening of amber suppressor tRNAs suitable for incorporation of non-natural amino acids in a cell-free translation system.PMID 18634752Read ↗

Development of amber suppressor tRNAs appropriate for incorporation of nonnatural amino acids.Semantic proximity Read ↗

In vitro selection of tRNAs for efficient four-base decoding to incorporate non-natural amino acids into proteins in an Escherichia coli cell-free translation system.Semantic proximity Read ↗

A tRNAModification-based strategy for Identifying amiNo acid Overproducers (AMINO).Semantic proximity Read ↗

Engineering of tRNAPro1E2 anticodon stem enhances multiple/consecutive ribosomal incorporation of N-methyl-l-α-amino acids and d-α-amino acids.Semantic proximity Read ↗

Two-Tier Screening Platform for Directed Evolution of Aminoacyl-tRNA Synthetases with Enhanced Stop Codon Suppression Efficiency.Semantic proximity Read ↗

Twenty-first aminoacyl-tRNA synthetase-suppressor tRNA pairs for possible use in site-specific incorporation of amino acid analogues into proteins in eukaryotes and in eubacteria.Semantic proximity Read ↗

Identification of permissive amber suppression sites for efficient non-canonical amino acid incorporation in mammalian cells.Semantic proximity Read ↗

Amber codon-mediated expanded saturation mutagenesis of proteins using a cell-free translation system.Semantic proximity Read ↗

Evolving Orthogonal Suppressor tRNAs To Incorporate Modified Amino Acids.Semantic proximity Read ↗

Performance analysis of orthogonal pairs designed for an expanded eukaryotic genetic code.Semantic proximity Read ↗

Evolving Escherichia coli to use a tRNA with a non-canonical fold as an adaptor of the genetic code.Semantic proximity Read ↗

The Pyrrolysyl-tRNA Synthetase Activity can be Improved by a P188 Mutation that Stabilizes the Full-Length Enzyme.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.