Article
Transfer RNA-mediated suppression of stop codons in protoplasts and transgenic plants.
Plant molecular biology - 1 Jul 1993
Carneiro V T, Pelletier G, Small I
Abstract excerpt
We have developed a simple, rapid and sensitive assay for tRNA gene expression in plant cells. A plant tRNA(Leu) gene was site-specifically mutated to encode each of the three anticodon sequences (CUA, UUA and UCA) that recognize, respectively, the amber, ochre and opal stop codons. The suppression activity of these genes was detected by their ability to restore transient beta-glucuronidase (GUS) expression in...
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