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Article

Amber suppression in mammalian cells dependent upon expression of an Escherichia coli aminoacyl-tRNA synthetase gene.

Molecular and cellular biology - 1 Mar 1996

Drabkin H J, Park H J, RajBhandary U L

Abstract excerpt

As an approach to inducible suppression of nonsense mutations in mammalian and in higher eukaryotic cells, we have analyzed the expression of an Escherichia coli glutamine-inserting amber suppressor tRNA gene in COS-1 and CV-1 monkey kidney cells. The tRNA gene used has the suppressor tRNA coding sequence flanked by sequences derived from a human initiator methionine tRNA gene and has two changes in the coding...

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Identifiers

PMID: 8622693
DOI: 10.1128/MCB.16.3.907
PMCID: PMC231072

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