Amber suppression in mammalian cells dependent upon expression of an Escherichia coli aminoacyl-tRNA synthetase gene.

doi:10.1128/MCB.16.3.907

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Article

Amber suppression in mammalian cells dependent upon expression of an Escherichia coli aminoacyl-tRNA synthetase gene.

Molecular and cellular biology - 1 Mar 1996

Drabkin H J, Park H J, RajBhandary U L

Abstract excerpt

As an approach to inducible suppression of nonsense mutations in mammalian and in higher eukaryotic cells, we have analyzed the expression of an Escherichia coli glutamine-inserting amber suppressor tRNA gene in COS-1 and CV-1 monkey kidney cells. The tRNA gene used has the suppressor tRNA coding...

Topics

Amino Acyl-tRNA Synthetases
Animals
Base Sequence
Cell Line
Codon, Nonsense
Escherichia coli
Gene Expression Regulation
Haplorhini
Molecular Sequence Data
Mutation
Plasmids
RNA, Transfer

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