Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Optimization of trans-splicing ribozyme efficiency and specificity by in vivo genetic selection.

Nucleic acids research - 15 Dec 2002

Ayre Brian G, Köhler Uwe, Turgeon Robert, Haseloff Jim

Abstract excerpt

Trans-splicing ribozymes are RNA-based catalysts capable of splicing RNA sequences from one transcript specifically into a separate target transcript. In doing so, a chimeric mRNA can be produced, and new gene activities triggered in living cells dependent on the presence of the target mRNA. Based on this ability of trans-splicing ribozymes to deliver new gene activities, a simple and versatile plating assay was...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Division
Share to Genetic Complementation Test
Share to Green Fluorescent Proteins

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Luminescent Proteins

Share to Recombinant Fusion Proteins

Share to RNA, Catalytic

Share to Substrate Specificity

Share to Transformation, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 12490732
DOI: 10.1093/nar/gnf141
PMCID: PMC140090

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Optimization of trans-splicing ribozyme efficiency and specificity by in vivo genetic selection.PMID 12490732Read ↗

Trans-splicing ribozymes for targeted gene delivery.Semantic proximity Read ↗

Evaluating and enhancing ribozyme reaction efficiency in mammalian cells.Semantic proximity Read ↗

A synthetic RNA-mediated evolution system in yeast.Semantic proximity Read ↗

A synthetic RNA-mediated evolution system in yeastSemantic proximity Read ↗

Gene therapy progress and prospects: reprograming gene expression by trans-splicing.Semantic proximity Read ↗

Incorporation of non-canonical amino acids into proteins in yeast.Semantic proximity Read ↗

Ribozyme gene therapy: applications for molecular medicine.Semantic proximity Read ↗

In vivo site-directed recombination (SDR): An efficient tool to reveal beneficial epistasis.Semantic proximity Read ↗

T7 Polymerase Expression of Guide RNAs in vivo Allows Exportable CRISPR-Cas9 Editing in Multiple Yeast Hosts.Semantic proximity Read ↗

Conversion of polyploid and alloploid Saccharomyces sensu stricto strains to leu2 mutants by genome DNA editing.Semantic proximity Read ↗

In vivo continuous evolution of genes and pathways in yeast.Semantic proximity Read ↗

RNAi-assisted genome evolution in Saccharomyces cerevisiae for complex phenotype engineering.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.