Article
Mutants of initiator tRNA that function both as initiators and elongators.
The Journal of biological chemistry - 25 Sept 1991
Varshney U, Lee C P, Seong B L, RajBhandary U L
Abstract excerpt
We describe the effect of mutations in the acceptor stem of Escherichia coli initiator tRNA on its function in vivo. The acceptor stem mutations were coupled to mutations in the anticodon sequence from CAU----CUA to allow functional studies on the mutant tRNAs in initiation and in elongation in v...
Topics
- Ampicillin
- Anticodon
- Base Sequence
- Blotting, Western
- Chloramphenicol
- Chloramphenicol O-Acetyltransferase
- Escherichia coli
- Kanamycin
- Molecular Sequence Data
- Mutation
- Plasmids
- RNA, Transfer
- beta-Lactamases
