Article
The functional analysis of nonsense suppressors derived from in vitro engineered Saccharomyces cerevisiae tRNA(Trp) genes.
Gene - 30 Nov 1993
Atkin A L, Riazi M A, Greer C L, Roy K L, Bell J B
Abstract excerpt
Nonsense suppressors derived from Saccharomyces cerevisiae tRNA(Trp) genes have not been identified by classical genetic screens, although one can construct efficient amber (am) suppressors from them by making the appropriate anticodon mutation in vitro. Herein, a series of in vitro constructed p...
Topics
- Base Sequence
- Blotting, Northern
- Genes, Fungal
- Genes, Suppressor
- Introns
- Molecular Sequence Data
- Nucleic Acid Conformation
- Phenotype
- RNA Processing, Post-Transcriptional
- RNA, Fungal
- RNA, Messenger
- RNA, Transfer, Trp
- Saccharomyces cerevisiae
- Transformation, Genetic