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Article

Targeted mutagenesis by homologous recombination in D. melanogaster.

Genes & development - 15 Jun 2002

Rong Yikang S, Titen Simon W, Xie Heng B, Golic Mary M, Bastiani Michael, Bandyopadhyay Pradip, Olivera Baldomero M, Brodsky Michael, Rubin Gerald M, Golic Kent G

Abstract excerpt

We used a recently developed method to produce mutant alleles of five endogenous Drosophila genes, including the homolog of the p53 tumor suppressor. Transgenic expression of the FLP site-specific recombinase and the I-SceI endonuclease generates extrachromosomal linear DNA molecules in vivo. These molecules undergo homologous recombination with the corresponding chromosomal locus to generate targeted alterations...

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Identifiers

PMID: 12080094
DOI: 10.1101/gad.986602
PMCID: PMC186348

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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