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Article

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast.

Journal of visualized experiments : JoVE - 26 Jan 2017

Duina Andrea A, Turkal Claire E

Abstract excerpt

We describe a PCR- and homologous recombination-based system for generating targeted mutations in histone genes in budding yeast cells. The resulting mutant alleles reside at their endogenous genomic sites and no exogenous DNA sequences are left in the genome following the procedure. Since in haploid yeast cells each of the four core histone proteins is encoded by two non-allelic genes with highly homologous open...

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PMID: 28190067
DOI: 10.3791/55263
PMCID: PMC5409331

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