Methods for homologous recombination in Drosophila.

Abstract excerpt

We present detailed protocols for two methods of gene targeting in Drosophila. The first, ends-out targeting, is identical in concept to gene replacement techniques used routinely in mammalian and yeast cells. In Drosophila, the targeted gene is replaced by the marker gene white + (although options exist to generate unmarked targeted alleles). This approach is simple in both the molecular cloning and the genetic...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Genetic Techniques
• Share to Mutagenesis
• Share to Cloning, Molecular