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Article

Creating recombination-activated genes and sequence-defined mutant libraries using transposons.

Methods in enzymology - 1 Jan 2007

Gallagher Larry, Turner Cheri, Ramage Elizabeth, Manoil Colin

Abstract excerpt

The properties of a collection of transposon Tn5 derivatives that generate reporter gene fusions and internal protein tags are summarized. Procedures utilizing several of the transposons for generating genes activated by Cre-loxP recombination and for creating large sequence-defined mutant libraries are described in detail.

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Identifiers

PMID: 17352920
DOI: 10.1016/S0076-6879(06)21012-7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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