Article
Electrospray tandem mass spectrometry of intact beta-chain hemoglobin variants.
Analytical chemistry - 1 May 2002
Rai Dilip K, Landin Britta, Alvelius Gunvor, Griffiths William J
Abstract excerpt
In this report, we present data to illustrate how human hemoglobin (Hb) variants can be identified by electrospray tandem mass spectrometry (MS/MS) of the intact Hb chains following the one-step dilution of whole blood. MS/MS spectra were recorded on a series of intact beta-chain human Hb variants. The resultant spectra were interpreted, and using the information gleaned from the fragmentation patterns of known...
Topics
- Genetic Variation
- Globins
- Hemoglobins
- Hemoglobins, Abnormal
- Humans
- Mutation
- Spectrometry, Mass, Electrospray Ionization