Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Automated identification of amino acid sequence variations in proteins by HPLC/microspray tandem mass spectrometry.

Analytical chemistry - 15 Feb 2000

Gatlin C L, Eng J K, Cross S T, Detter J C, Yates J R

Abstract excerpt

Amino acid sequence variations resulting from single-nucleotide polymorphisms (SNPs) were identified using a novel mass spectrometric method. This method obtains 99+% protein sequence coverage for human hemoglobin in a single LC-microspray tandem mass spectrometry (microLC-MS/MS) experiment. Tandem mass spectrometry data was analyzed using a modified version of the computer program SEQUEST to identify the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acid Sequence
Share to Chromatography, High Pressure Liquid

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Hemoglobins

Share to Mass Spectrometry

Share to Molecular Sequence Data

Share to Polymorphism, Genetic

Share to Sequence Analysis, Protein

Share to Sequence Homology, Amino Acid

Source

Identifiers

PMID: 10701260
DOI: 10.1021/ac991025n

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Automated identification of amino acid sequence variations in proteins by HPLC/microspray tandem mass spectrometry.PMID 10701260Read ↗

Mass spectrometry: a tool for enhanced detection of hemoglobin variants.Semantic proximity Read ↗

Advanced analytical methods for hemoglobin variants.Semantic proximity Read ↗

Automated analysis of hemoglobin variants using nanoLC-MS and customized databases.Semantic proximity Read ↗

Rapid identification of hemoglobin variants by electrospray ionization mass spectrometry.Semantic proximity Read ↗

HPLC-ESI-MS/MS analysis of hemoglobin peptides in tryptic digests of dried-blood spot extracts detects HbS, HbC, HbD, HbE, HbO-Arab, and HbG-Philadelphia mutations.Semantic proximity Read ↗

Optimization and Identification of Single Mutation in Hemoglobin Variants with 2,2,2 Trifluoroethanol Modified Digestion Method and Nano-LC Coupled MALDI MS/MS.Semantic proximity Read ↗

Advances in hemoglobinopathy detection and identification.Semantic proximity Read ↗

New approach for rapid detection of known hemoglobin variants using LC-MS/MS combined with a peptide database.Semantic proximity Read ↗

Peptide mapping using thermospray LC/MS detection: rapid identification of hemoglobin variants.Semantic proximity Read ↗

In silico based Collision Energy optimization for targeted MRM assay of Hemoglobin variants using SkylineSemantic proximity Read ↗

Hemoglobin variant analysis of whole blood and dried blood spots by MS.Semantic proximity Read ↗

Identification and characterization by high-performance liquid chromatography/electrospray ionization mass spectrometry of a new variant hemoglobin, Mataro [beta134(H12) Val > Ala.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.