Article
Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation.
Nucleic acids research - 15 Feb 2002
Yang Yee Hwa, Dudoit Sandrine, Luu Percy, Lin David M, Peng Vivian, Ngai John, Speed Terence P
Abstract excerpt
There are many sources of systematic variation in cDNA microarray experiments which affect the measured gene expression levels (e.g. differences in labeling efficiency between the two fluorescent dyes). The term normalization refers to the process of removing such variation. A constant adjustment is often used to force the distribution of the intensity log ratios to have a median of zero for each slide. However,...
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