Article
Purification and characterization of a mutant DnaB protein specifically defective in ATP hydrolysis.
Journal of bacteriology - 1 Dec 1992
Shrimankar P, Stordal L, Maurer R
Abstract excerpt
The dnaB gene of Escherichia coli encodes an essential DNA replication enzyme. Fueled by the energy derived from the hydrolysis of ATP to ADP+P(i), this enzyme unwinds double-stranded DNA in advance of the DNA polymerase. While doing so, it intermittently stimulates primase to synthesize an RNA p...
Topics
- Adenosine Triphosphate
- Arginine
- Bacterial Proteins
- Cloning, Molecular
- Cysteine
- DNA Helicases
- DNA Primase
- DNA Replication
- DnaB Helicases
- Escherichia coli
- Genes, Dominant
- Genes, Lethal
- Hydrolysis
- Mutation
- RNA Nucleotidyltransferases