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Article

Purification and characterization of a mutant DnaB protein specifically defective in ATP hydrolysis.

Journal of bacteriology - 1 Dec 1992

Shrimankar P, Stordal L, Maurer R

Abstract excerpt

The dnaB gene of Escherichia coli encodes an essential DNA replication enzyme. Fueled by the energy derived from the hydrolysis of ATP to ADP+P(i), this enzyme unwinds double-stranded DNA in advance of the DNA polymerase. While doing so, it intermittently stimulates primase to synthesize an RNA primer for an Okazaki fragment. To better understand the structural basis of these and other aspects of DnaB function,...

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Identifiers

PMID: 1332941
DOI: 10.1128/jb.174.23.7689-7696.1992
PMCID: PMC207482

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