Article
Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution.
Protein engineering - 1 Sept 2001
Sun L, Petrounia I P, Yagasaki M, Bandara G, Arnold F H
Abstract excerpt
We have used directed evolution methods to express a fungal enzyme, galactose oxidase (GOase), in functional form in Escherichia coli. The evolved enzymes retain the activity and substrate specificity of the native fungal oxidase, but are more thermostable, are expressed at a much higher level (up to 10.8 mg/l of purified GOase), and have reduced negative charge compared to wild type, all properties which are...
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