Article
Structural and kinetic studies of a series of mutants of galactose oxidase identified by directed evolution.
Protein engineering, design & selection : PEDS - 1 Feb 2004
Wilkinson D, Akumanyi N, Hurtado-Guerrero R, Dawkes H, Knowles P F, Phillips S E V, McPherson M J
Abstract excerpt
Galactose oxidase (GO; E.C. 1.1.3.9) is a copper- containing enzyme that oxidizes a range of primary alcohols to aldehydes. This broad substrate specificity is reflected in a high K(M) for substrates. Directed evolution has previously been used to select variants of GO that exhibit enhanced expression and kinetic properties. In assays using unpurified enzyme samples, the variant C383S displayed a 5-fold lower...
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