Article
Focused Directed Evolution of Aryl-Alcohol Oxidase in Saccharomyces cerevisiae by Using Chimeric Signal Peptides.
Applied and environmental microbiology - 1 Sept 2015
Viña-Gonzalez Javier, Gonzalez-Perez David, Ferreira Patricia, Martinez Angel T, Alcalde Miguel
Abstract excerpt
Aryl-alcohol oxidase (AAO) is an extracellular flavoprotein that supplies ligninolytic peroxidases with H2O2 during natural wood decay. With a broad substrate specificity and highly stereoselective reaction mechanism, AAO is an attractive candidate for studies into organic synthesis and synthetic biology, and yet the lack of suitable heterologous expression systems has precluded its engineering by directed...
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