Article
Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast.
Microbial cell factories - 18 Mar 2010
García-Ruiz Eva, Maté Diana, Ballesteros Antonio, Martinez Angel T, Alcalde Miguel
Abstract excerpt
BACKGROUND: In the picture of a laboratory evolution experiment, to improve the thermostability whilst maintaining the activity requires of suitable procedures to generate diversity in combination with robust high-throughput protocols. The current work describes how to achieve this goal by engine...
Topics
- Basidiomycota
- Directed Molecular Evolution
- Enzyme Stability
- Fungal Proteins
- Gene Expression
- Mutation
- Oxidoreductases
- Protein Engineering
- Saccharomyces cerevisiae