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Article

Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast.

Microbial cell factories - 18 Mar 2010

García-Ruiz Eva, Maté Diana, Ballesteros Antonio, Martinez Angel T, Alcalde Miguel

Abstract excerpt

BACKGROUND: In the picture of a laboratory evolution experiment, to improve the thermostability whilst maintaining the activity requires of suitable procedures to generate diversity in combination with robust high-throughput protocols. The current work describes how to achieve this goal by engineering ligninolytic oxidoreductases (a high-redox potential laccase -HRPL- and a versatile peroxidase, -VP-)...

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Identifiers

PMID: 20298573
DOI: 10.1186/1475-2859-9-17
PMCID: PMC2847542

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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