Article
Construction of mutant glucose oxidases with increased dye-mediated dehydrogenase activity.
International journal of molecular sciences - 2 Nov 2012
Horaguchi Yohei, Saito Shoko, Kojima Katsuhiro, Tsugawa Wakako, Ferri Stefano, Sode Koji
Abstract excerpt
Mutagenesis studies on glucose oxidases (GOxs) were conducted to construct GOxs with reduced oxidase activity and increased dehydrogenase activity. We focused on two representative GOxs, of which crystal structures have already been reported—Penicillium amagasakiense GOx (PDB ID; 1gpe) and Aspergillus niger GOx (PDB ID; 1cf3). We constructed oxygen-interacting structural models for GOxs, and predicted...
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