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Colorimetric High-Throughput Screening Assays for the Directed Evolution of Fungal Laccases.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018

Pardo Isabel, Camarero Susana

Abstract excerpt

In this chapter we describe several high-throughput screening assays for the evaluation of mutant libraries for the directed evolution of fungal laccases in the yeast Saccharomyces cerevisiae. The assays are based on the direct oxidation of three syringyl-type phenols derived from lignin (sinapic acid, acetosyringone, and syringaldehyde), an artificial laccase mediator (violuric acid), and three organic synthetic...

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PMID: 29086313
DOI: 10.1007/978-1-4939-7366-8_14

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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