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Article

Glycoprotein labeling using engineered variants of galactose oxidase obtained by directed evolution.

Journal of the American Chemical Society - 8 Jun 2011

Rannes Julie B, Ioannou Avgousta, Willies Simon C, Grogan Gideon, Behrens Carsten, Flitsch Sabine L, Turner Nicholas J

Abstract excerpt

A directed evolution approach has been used for the generation of variants of galactose oxidase (GOase) that can selectively oxidize glycans on glycoproteins. The aldehyde function introduced on the glycans D-mannose (Man) and D-N-acetyl glucosamine (GlcNAc) by the enzyme variants could then be used to label the glycoproteins and also whole cells that display mannosides on their surface.

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Source

Identifiers

PMID: 21526835
DOI: 10.1021/ja2018477

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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