Article
Directed evolution of glucose oxidase from Aspergillus niger for ferrocenemethanol-mediated electron transfer.
Biotechnology journal - 1 Feb 2007
Zhu Ziwei, Wang Mian, Gautam Amit, Nazor Jovana, Momeu Carmen, Prodanovic Radivoje, Schwaneberg Ulrich
Abstract excerpt
A directed evolution protocol was developed for glucose oxidase (GOx) from Aspergillus niger that mimics applications conditions and employs a well-known mediator, oxidized ferrocenemethanol, in a medium throughput screen (96-well plate format). Upon reduction, oxidized ferrocenemethanol shows a color change from blue to pale yellow that can be recorded at 625 nm. Under optimized screening conditions, a CV of...
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