Article
Application of a very high-throughput digital imaging screen to evolve the enzyme galactose oxidase.
Protein engineering - 1 Apr 2001
Delagrave S, Murphy D J, Pruss J L, Maffia A M, Marrs B L, Bylina E J, Coleman W J, Grek C L, Dilworth M R, Yang M M, Youvan D C
Abstract excerpt
Directed evolution has become an important enabling technology for the development of new enzymes in the chemical and pharmaceutical industries. Some of the most interesting substrates for these enzymes, such as polymers, have poor solubility or form highly viscous solutions and are therefore refractory to traditional high-throughput screens used in directed evolution. We combined digital imaging spectroscopy and...
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