Article
Arrest of cell division and nucleoid partition by genetic alterations in the sliding clamp of the replicase and in DnaA.
Molecular genetics and genomics : MGG - 1 Oct 2001
Kawakami H, Iwura T, Takata M, Sekimizu K, Hiraga S, Katayama T
Abstract excerpt
In Escherichia coli, an interaction between the replication initiator DnaA and the sliding clamp protein, the beta subunit (DnaN) of DNA polymerase III, is required to regulate the chromosomal replication cycle. We report here that colony formation by, and cell division of, the temperature (42 degrees C)-sensitive dnaN59 mutant are inhibited at 34-35 degrees C when DnaA is moderately (4-to 8-fold ) overexpressed,...
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