Article
Growth phase dependent changes in the structure and protein composition of nucleoid in Escherichia coli.
Science China. Life sciences - 1 Sept 2015
Talukder AliAzam, Ishihama Akira
Abstract excerpt
The genomic DNA of bacteria is highly compacted in a single or a few bodies known as nucleoids. Here, we have isolated Escherichia coli nucleoid by sucrose density gradient centrifugation. The sedimentation rates, structures as well as protein/ DNA composition of isolated nucleoids were then compared under various growth phases. The nucleoid structures were found to undergo changes during the cell growth; i. e.,...
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