Article
A novel yeast system for in vivo selection of recognition sequences: defining an optimal c-Myb-responsive element.
Nucleic acids research - 15 Oct 2001
Berge T, Bergholtz S L, Andersson K B, Gabrielsen O S
Abstract excerpt
Yeast (Saccharomyces cerevisiae) has proved to be a highly valuable tool in a range of screening methods. We present in this work the design and use of a novel yeast effector-reporter system for selection of sequences recognised by DNA-binding proteins in vivo. A dual HIS3-lacZ reporter under the control of a single randomised response element facilitates both positive growth selection of binding sequences and...
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