Article
Identification of transcription factor targets by phenotypic activation and microarray expression profiling in yeast.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009
Chua Gordon
Abstract excerpt
A major obstacle to identify physiological transcriptional targets is that the conditions that induce the majority of yeast transcription factors (TFs) are unknown. Microarray analyses of deletion mutants indicate that most TFs are inactive under standard growth conditions. To overcome this, we screened an ordered array of yeast open reading frames (ORFs) to identify TFs that confer reduced fitness upon...
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