Article
Yeast regulatory protein LEU3: a structure-function analysis.
Nucleic acids research - 25 Jan 1990
Zhou K M, Bai Y L, Kohlhaw G B
Abstract excerpt
Eleven mutations resulting in partially deleted or truncated LEU3 protein were generated by linker insertion or other modifications at restriction sites, deletion of restriction fragments, or oligonucleotide-directed mutagenesis. Functional studies of these mutants showed the following: (i) A specific DNA binding region is contained within the 173 N-terminal residues, but other regions of the protein are required...
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