Article
The activation specificities of wild-type and mutant Gcn4p in vivo can be different from the DNA binding specificities of the corresponding bZip peptides in vitro.
Journal of molecular biology - 13 Mar 1998
Suckow M, Hollenberg C P
Abstract excerpt
Single amino acid substitutions which previously have been shown to alter the DNA binding specificity of a Gcn4p bZip peptide in vitro were transformed to full length Gcn4p, and activation of a test promoter carrying various palindromic and pseudo-palindromic binding sites was measured. All mutat...
Topics
- Amino Acid Sequence
- Amino Acid Substitution
- Base Sequence
- Basic-Leucine Zipper Transcription Factors
- Binding Sites
- DNA, Fungal
- DNA-Binding Proteins
- Fungal Proteins
- G-Box Binding Factors
- Gene Expression Regulation, Fungal
- Genes, Reporter
- Leucine Zippers
- Mutation
- Phenotype
- Protein Kinases
- Repressor Proteins
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins