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Article

Use of a reporter gene assay in yeast for genetic analysis of DNA-protein interactions.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009

Setzer David R, Schulman Deborah B, Gunther Cathy V, Bumbulis Michael J

Abstract excerpt

We describe methods for the genetic analysis of a DNA-protein interaction from any species. The DNA-binding domain of the protein of interest is expressed in yeast cells as a fusion with a known transcriptional activation domain, and the target binding site is used as an artificial upstream activation sequence (UAS) in an engineered promoter driving expression of a reporter gene, such as beta-galactosidase....

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PMID: 19378169
DOI: 10.1007/978-1-60327-015-1_15

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