Production of a heterozygous mutant cell line by homologous recombination (single knockout).

Abstract excerpt

Gene targeting by homologous recombination is a powerful and widely used technique for introduction of specific gene mutations (frequently a gene inactivation) in transgenic animals. The basic method detailed in this unit uses sequences homologous to the endogenous gene flanking the mutation. While methods using bacterial artificial chromosomes (BACs) and recombineering may be used, in most cases simpler...

Topics

• Cell Line
• Heterozygote
• Mutation
• Recombination, Genetic