Article
The DT40 web site: sampling and connecting the genes of a B cell line.
Nucleic acids research - 1 Jan 2002
Buerstedde Jean-Marie, Arakawa Hiroshi, Watahiki Akira, Carninci Piere Piero, Hayashizaki Y Yoshihide, Korn Bernhard, Plachy Jiri
Abstract excerpt
Thousands of new vertebrate genes have been discovered and genetic systems are needed to address their functions at the cellular level. The chicken B cell line DT40 allows efficient gene disruptions due to its high homologous recombination activity. However, cloning the gene of interest is often cumbersome, since relatively few chicken cDNA sequences are present in the public databases. In addition, the...
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