Article
Modular construction of extended DNA recognition surfaces: mutant DNA-binding domains of the 434 repressor as building blocks.
Protein engineering - 1 Aug 2001
Liang T, Chen J, Tjörnhammar M L, Pongor S, Simoncsits A
Abstract excerpt
Single-chain derivatives of the 434 repressor containing one wild-type and one mutant DNA-binding domain recognize the general operator ACAA-6 base pairs-NNNN, where the ACAA operator subsite is contacted by the wild-type and the NNNN tetramer by the mutant domain. The DNA-binding specificities of several single-chain mutants were studied in detail and the optimal subsites of the mutant domains were determined....
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