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Article

Isolation of altered specificity mutants of the single-chain 434 repressor that recognize asymmetric DNA sequences containing the TTAA and TTAC subsites.

Nucleic acids research - 1 Sept 1999

Simoncsits A, Tjörnhammar M L, Wang S, Pongor S

Abstract excerpt

A novel single-chain (sc) protein framework containing covalently dimerized DNA-binding domains (DBD) of the phage 434 repressor was used to construct combinatorial mutant libraries in order to isolate mutant DBDs with altered specificities. The library members contain one wild-type DBD and one mutant domain with randomized amino acids in the DNA-contacting region. Based on previous studies, the mutant sc...

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Identifiers

PMID: 10446235
DOI: 10.1093/nar/27.17.3474
PMCID: PMC148589

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