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Article

Repertoire selection of variant single-chain Cro: toward directed DNA-binding specificity of helix-turn-helix proteins.

Biochemistry - 28 Sept 2004

Nilsson Mikael T I, Widersten Mikael

Abstract excerpt

A single-chain derivative of the lambda Cro repressor (scCro) has been randomly mutated in amino acid residues critical for specific DNA recognition to create libraries of protein variants. Utilizing phage display-afforded affinity selection, scCro variants have been isolated for binding to synthetic DNA ligands. Isolated scCro variants were analyzed functionally, both in fusion with phage particles and after...

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Identifiers

PMID: 15379544
DOI: 10.1021/bi049122k

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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